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FOXO4-DRI has been investigated extensively in preclinical academic literature for p53 transactivation domain binding, senescence-associated apoptosis pathway research, p16 and IL-6 marker dynamics in senescent cell research models, and SASP (senescence-associated secretory phenotype) signaling

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The cells were then permeabilized and blocked using 0.5% Triton X-100 and 5% BSA, respectively and then incubated with anti-NOX2 primary antibody (1:500, Proteintech) at 4 C overnight and with fluorescence dye-conjugated secondary antibodies at 37 C 1 h